Composite

Part:BBa_K2637048:Design

Designed by: Jiangnan Xie   Group: iGEM18_Tianjin   (2018-10-05)


Fluc with TDH3 Promoter and ADH1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1119
    Illegal NgoMIV site found at 2463
    Illegal NgoMIV site found at 2484
    Illegal AgeI site found at 2187
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2369


Design Notes

We connected Fluc witth the constitutive TDH3 promoter and ADH1 terminator to make the cassette which can express Fluc continuously.


Source

The sequence of Fluc is already published by Promega [http://www.promega.com/vectors/pgl4.10.txt here]. The TDH3 promoter and ADH1 terminator are from saccharomyces cerevisiae.


References